SICKLE CELL SCREEN WITH HEMOGLOBIN EVALUATION IF INDICATED.

Code
000.0000
Name
SICKLE CELL SCREEN WITH HEMOGLOBIN EVALUATION IF INDICATED.
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
CPT Codes
85660; If indicated, add 83021
Site
SBMF
Reference Test
25170
ATLAS Test Code

Specimen Information

Type

Lavender, EDTA, WHOLE BLOOD

Volume

5.0 ml

Transport Info

Refrigerated

Fasting Required?
False
Patient Instructions

Reference Range

See Report

Methodology

Hemoglobin S Solubility
High Performance Liquid Chromatography (HPLC)

Clinical Significance

Detect sickling hemoglobins.

The sickling reaction is based on the relative insolubility of hemoglobin S (HbS). Erythrocytes are lysed by saponin and the released hemoglobin is reduced by sodium dithionite in a phosphate buffer. Its very low solubility and the formation of threadlike liquid crystals characterize reduced HbS, so that in the presence of HbS or non-sickling hemoglobins the system becomes turbid. Other normal hemoglobins lyse with no crystallization and will form a transparent solution. Electrophoretic confirmation is required for conclusive identification in either case.

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