SICKLE CELL SCREEN WITH HEMOGLOBIN EVALUATION IF INDICATED.
- Code
- 000.0000
- Name
- SICKLE CELL SCREEN WITH HEMOGLOBIN EVALUATION IF INDICATED.
- Category
- None
- Department
- Send-Out
- Start Date
- Expiration Date
- Synonyms
- CPT Codes
- 85660; If indicated, add 83021
- Site
- SBMF
- Reference Test
- 25170
Specimen Information
- Type
Lavender, EDTA, WHOLE BLOOD
- Volume
5.0 ml
- Transport Info
Refrigerated
- Fasting Required?
- False
- Patient Instructions
- Reference Range
See Report
- Methodology
Hemoglobin S Solubility
High Performance Liquid Chromatography (HPLC)
Clinical Significance
Detect sickling hemoglobins.
The sickling reaction is based on the relative insolubility of hemoglobin S (HbS). Erythrocytes are lysed by saponin and the released hemoglobin is reduced by sodium dithionite in a phosphate buffer. Its very low solubility and the formation of threadlike liquid crystals characterize reduced HbS, so that in the presence of HbS or non-sickling hemoglobins the system becomes turbid. Other normal hemoglobins lyse with no crystallization and will form a transparent solution. Electrophoretic confirmation is required for conclusive identification in either case.