B-CELL AND T-CELL COMPREHENSIVE CLONALITY ASSESSMENT

Code
000.0000
Name
B-CELL AND T-CELL COMPREHENSIVE CLONALITY ASSESSMENT
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
B-Cell and T-Cell receptor gene rearrangement
CPT Codes
83891; 83900; 83901x14; 83909x10; 83912-26
Site
SBMF
Reference Test
36168
ATLAS Test Code

Specimen Information

Type

Lavender top (EDTA) tube, WHOLE BLOOD or BONE MARROW

Tissue: Formalin-fixed paraffin embedded tissue block, OR Tissue fixed in 10% buffered formalin

Volume

Whole blood: 5.0 mL
Bone marrow: 3.0 mL
Tissue: 50 mg

Transport Info

Special Instructions--Call Lab

Fasting Required?
False
Patient Instructions

Reference Range

No monoclonal B cell population was detected by PCR analysis
No monoclonal T cell population was detected by PCR analysis

Methodology

Polymerase Chain Reaction (PCR)

Clinical Significance

Most malignant lymphomas are readily identifiable by laboratory methods such as histology and immunohistochemistry and/or flow cytometry. There are some lymphoproliferative lesions, however, where the abnormal lymphocytes are difficult to identify by routine histology. The clonal lymphocytes may be few in number within the tissue, or possibly dispersed within a background of polyclonal “reactive” lymphocytes or other inflammatory cells. Existing immunochemical markers may not readily identify the lymphocytes as malignant cells, a phenomenon that can occur in some T cell neoplasms. In such cases, identification of a genetically abnormal, clonal T or B lymphocyte population can be key to establishing the correct histopathologic diagnosis.

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