BILIRUBIN, AMNIOTIC FLUID
- BILIRUBIN, AMNIOTIC FLUID
- Start Date
- Expiration Date
- Amniotic Fluid Bilirubin; OD 450
- CPT Codes
- Reference Test
Amniotic fluid, Light-proof (amber) plastic container with screw-top cap
- Transport Info
Light-proof (amber) plastic container with screw-top cap
- Fasting Required?
- Patient Instructions
- Reference Range
Normal: 0.02 OD or less; depends on gestational age.
Predictor of the severity of hemolytic disease.
Spectrophotometric analysis of amniotic fluid in Rh-sensitized pregnancies shows an abnormal peak at 450 nm caused by bilirubin and several related bilirubinoid pigments. The magnitude of the increase in absorbance above the baseline value usually correlates well, for any gestational age, with the intensity of the hemolytic disease. The higher the DA450, in relation to the gestational age, the more severe the hemolytic disease. Generally, a decreasing amniotic fluid bilirubin trend indicates a fetus that will survive, and a horizontal or rising bilirubin indicates that there is severe erythroblastosis fetalis. Although a single amniotic fluid analysis from a 28-32 week gestation can be predictable of the severity of hemolytic disease, multiple sequential determinations are most often necessary.