DIRECT COOMBS
- Code
- 120.1800
- Name
- DIRECT COOMBS
- Category
- None
- Department
- Blood Bank
- Start Date
- Expiration Date
- Synonyms
- DAT
- CPT Codes
- 86880
- Site
- Main Lab
- Reference Test
Specimen Information
- Type
Pink,EDTA
- Volume
6.0mL
- Transport Info
Refrigerated
- Fasting Required?
- False
- Patient Instructions
- Reference Range
Negative
- Methodology
Antigen-Antibody Reaction
Clinical Significance
It is commonly used in the diagnosis of hemolytic disease of the newborn, autoimmune hemolytic anemia, drug-induced red cell sensitization, and for investigation of transfusion reactions.
The direct antiglobulin (Coombs) test is a one-stage test for the detection of in vivo red cell sensitization. It is commonly used in the diagnosis of hemolytic disease of the newborn, autoimmune hemolytic anemia, drug-induced red cell sensitization, and for investigation of transfusion reactions.In this test, the cells under study are washed thoroughly with saline to remove any unattached serum globulins. The washed cells are then tested with polyspecific antihuman globulin (AHG) serum.Depending on the type of specimen used, a positive reaction indicates the presence of antibodies and/or complement attached to the cell surface.