ENTAMOEBA HISTOLYTICA IGG ANTIBODY BY ELISA

Code
000.0000
Name
ENTAMOEBA HISTOLYTICA IGG ANTIBODY BY ELISA
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
Amebic Ab, Amebiasis, Amoeba Histolytica
CPT Codes
86753
Site
SBMF
Reference Test
44303
ATLAS Test Code

Specimen Information

Type

Gold, SST

Volume

1.0 ml

Transport Info

Centrifuge and immediately transfer serum to separate plastic tube
Refrigerated

Fasting Required?
False
Patient Instructions

Reference Range

0.8 IV or less: Negative – No significant level of detectable E. histolytica IgG antibody
0.9-1.1 IV: Equivocal – Repeat testing in 10-14 days may be helpful
1.2 IV or greater: Positive – IgG antibody to E. histolytica detected, suggestive of a current or past infection

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.

Methodology

Enzyme-Linked Immunosorbent Assay

Clinical Significance

Entamoeba histolytica gives rise to serologically detectable specific antibodies when human or animal tissues are invaded. In recent years, a number of serologic tests have been developed to detect antibodies produced in response to tissue invasion by E. histolytica (amoebic liver abscess and, to a lesser extent, suspected amoebic colitis). These methods appear to be highly specific for amoebic antibodies, but vary in sensitivity dependent upon the type of infection. Titers are elevated in 100 percent of patients with amoebic liver abscesses, 98 percent with amoebic dysentery, and 66 percent of asymptomatic amoebic cyst passers. Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. In the case of extraintestinal complications, a positive antibody can indicate amebiasis even though stool findings are negative.

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