HEPARIN ASSAY

Code
000.0000
Name
HEPARIN ASSAY
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
Heparin Xa Assay, Unfractionated
CPT Codes
85520
Site
SBMF
Reference Test
25186
ATLAS Test Code

Specimen Information

Type

Blue, Citrate
Platelet-poor plasma (PPP) samples obtained by double centrifugation are critical for accurate coagulation studies as platelet contamination may cause spurious results

Volume

2.0 mL (Two aliquots, 1.0 mL each)

Transport Info

Separate plasma from cells immediately
• Promptly centrifuge 15 minutes
• Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
• Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
• Use second plastic pipette to carefully transfer PPP sample into plastic aliquot tubes
Frozen

Fasting Required?
False
Patient Instructions

Reference Range

Therapeutic range: 0.3-0.7 U/mL

Methodology

Spectrophotometry

Clinical Significance

Heparin by itself exhibits little anticoagulant activity. It requires a plasma cofactor, Antithrombin III, for its anticoagulant activity. Specifically, heparin is a catalyst that accelerates the neutralization of activated clotting factors such as Factor Xa and thrombin. The inhibition of Factor Xa by Antithrombin III is accelerated by heparin. Under optimum conditions the amount of Factor Xa activity neutralized during a predetermined time period is directly proportional to the concentration of heparin in the reaction mixture.

Back

Patients & Visitors

Make a First Appointment Maternity Pre-Registration Make a Donation Recognize a Sarah Bush Lincoln Employee