HEPARIN, LOW MOLECULAR WEIGHT

Code
000.0000
Name
HEPARIN, LOW MOLECULAR WEIGHT
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
CPT Codes
85520
Site
SBMF
Reference Test
25286
ATLAS Test Code

Specimen Information

Type

Blue, Citrate
Platelet-poor plasma (PPP) samples obtained by double centrifugation are critical for accurate coagulation studies as platelet contamination may cause spurious results

Volume

2.0 mL (Two aliquots, 1.0 mL each)

Transport Info

Separate plasma from cells immediately
• Promptly centrifuge 15 minutes
• Carefully transfer plasma portion of sample to separate plastic tube using plastic pipette
• Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP)
• Use second plastic pipette to carefully transfer PPP sample into 2 plastic aliquot tubes
Frozen

Fasting Required?
False
Patient Instructions

Separate plasma from cells immediately - Promptly, centrifuge 15 minutes - Immediately carefully transfer plasma portion of sample to separate plastic tube using plastic pipette Note: Buffy coat layer of sample must be avoided - Centrifuge transferred plasma sample again to produce platelet-poor plasma (PPP) - Use second plastic pipette to carefully transfer at least 0.5 mL PPP into each of 2 plastic aliquot tubes (total sample volume: at least 1.0 mL) - Within 4 hours of collection, freeze both PPP samples.

Reference Range

Therapeutic range varies with the type and manufacturer.
0.5-1.1 U/mL based on Lovenox (enoxaparin sodium).

Methodology

Spectrophotometry

Clinical Significance

Heparin by itself exhibits little anticoagulant activity. It requires a plasma cofactor, Antithrombin III, for its anticoagulant activity. Specifically, heparin is a catalyst that accelerates the neutralization of activated clotting factors such as Factor Xa and thrombin.The inhibition of Factor Xa by Antithrombin III is accelerated by heparin. Under optimum conditions the amount of Factor Xa activity neutralized during a predetermined time period is directly proportional to the concentration of heparin in the reaction mixture.

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