HEPATITIS C RNA (DNA PCR)

Code
900.1800
Name
HEPATITIS C RNA (DNA PCR)
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
HCV Viral load; HCV RNA Quantitation by PCR; HCV RNA by PCR
CPT Codes
87522
Site
SBMF
Reference Test
25106
ATLAS Test Code

Specimen Information

Type

Two (2) Pearl white top (PPT; EDTA + gel barrier) tubes

Volume

3.0 ml

Transport Info

Refrigerated

Fasting Required?
False
Patient Instructions

Reference Range

No HCV RNA detected.

Methodology

Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Clinical Significance

Assess viral response to antiviral treatment in conjunction with clinical presentation and other laboratory markers. The test is for research use only. It is not intended for use as a screening test or as a diagnostic test to confirm the presence of HCV infection. It should not be used without confirmation of the diagnosis by another medically established diagnostic product or procedure.

Hepatitis C Virus is considered to be the principle etiologic agent responsible for 90-95% of the cases of post-transfusion non-A and non-B hepatitis. HCV is a single-stranded, positive sense RNA virus with a genome of approximately 10,000 nucleotides coding for 3,000 amino acids. Hepatitis C infection is highly prevalent among patients receiving organ transplants, blood transfusions, or commercial clotting factors, as well as persons involved in intravenous drug abuse and those undergoing renal dialysis. At the present time, an immunological assay for the direct detection of HCV antigen is unavailable. Also, direct detection by viral culture methods is also not available. Therefore, there is no method currently available to provide a quantitative measure of HCV viremia. Detection and quantification of HCV viremia by Polymerase Chain Reaction (PCR) offers a way to measure active viremia. It is possible, using PCR, to detect HCV viremia prior to immunological seroconversion, and to detect fluctuation of viremia in antibody-positive chronic HCV patients undergoing therapy with interferon.

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