INSULIN, FREE & TOTAL

Code
600.6325
Name
INSULIN, FREE & TOTAL
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
CPT Codes
83525; 83527
Site
SBMF
Reference Test
30072
ATLAS Test Code

Specimen Information

Type

Gold, SST

Volume

2.0 ml

Transport Info

Centrifuge and immediately transfer serum to separate plastic tube
Frozen

Fasting Required?
False
Patient Instructions

Reference Range

Insulin, Total: 2.6-24.9 µU/mL
Insulin, Free: Less than 25 µU/mL

Methodology

Electrochemiluminescent Immunoassay (ECLIA)

Clinical Significance

Used to estimate active insulin concentrations in patients who have developed insulin antibodies

Patients on insulin therapy can develop antibodies to insulin thus binding the free insulin and inactivating it. Therefore, the measurement of free insulin is necessary to estimate active insulin concentrations in these patients.

Many diabetic patients develop insulin resistance after insulin treatment, due to the formation of insulin antibodies. When present in sufficient quantities, these antibodies can cause resistance to insulin or stimulate the release of insulin at inappropriate times. Knowledge of insulin antibodies is important when a change in source of insulin is contemplated, or when the patient is not responding predictably to insulin therapy.

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