LIPASE

Code
400.4151
Name
LIPASE
Category
None
Department
Chemistry
Start Date
Expiration Date
Synonyms
CPT Codes
83690
Site
Main Lab
Reference Test
ATLAS Test Code

Specimen Information

Type

Gold, SST

Volume

1.0 ml

Transport Info

Room Temperature
1 week stability

Refrigerated
1 week stability

Frozen
1 year stability

Fasting Required?
False
Patient Instructions

Reference Range

7-60 U/L

Methodology

Enzymatic

Clinical Significance

Used for the diagnosis of acute pancreatitis.

Lipases are glycoproteins with a molecular weight of 47000 daltons. They are defined as triglyceride hydrolases that catalyze the cleavage of triglycerides to diglycerides with subsequent formation of monoglycerides and fatty acids. In addition to alpha-amylase, pancreatic lipases have for many years been undeniably the most important clinical chemistry parameters for the differential diagnosis of diseases of the pancreas. The lipase activity determination has gained increasing international recognition because of its highly specificity and rapid response. After acute pancreatitis the lipase activity increases within 4-8 hours, reaches a peak after 24 hours and decreases after 8 to 14 days. However, There is no correlation between the lipase activity determined in serum and the extent of damage to the pancreas. Numerous methods have been described for the determination of lipase that determine the decrease in substrate turbidimetrically or nephelometrically, or determine degradation products.This method is based on the cleavage of a specific chromogenic lipase substrate 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester emulsified with bile acids. The pancreatic enzyme activity is determined specifically by the combination of bile acid and colipase used in this assay. Virtually no lipase activity is detected in the absence of colipase. Colipase only activates pancreatic lipase, but not other lipolytic enzymes found in serum. The high amount of cholates ensure that the esterases present in the serum do not react with the chromogenic substrate due to the highly negative surface charge.

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