PLATELET AB, INDIRECT

Code
900.3525
Name
PLATELET AB, INDIRECT
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
Antiplatelet, Circulating Platelet Antibodies
CPT Codes
86022
Site
SBMF
Reference Test
44477
ATLAS Test Code

Specimen Information

Type

Red, Plain

Volume

1.0 ml

Transport Info

Centrifuge and immediately transfer serum to separate plastic tube
Frozen

Fasting Required?
False
Patient Instructions

Reference Range

None detected.

Methodology

Enzyme-Linked Immunosorbent Assay (ELISA)

Clinical Significance

This is the primary test for detection of platelet-specific antibodies. It is not recommended for the diagnosis of immune thrombocytopenic purpura (ITP). This test will detect both allo and autoantibodies, but will not distinguish between them. Results of this test should be used in conjunction with clinical findings and other serological tests. Antibodies directed to antigens found on platelets are associated with many different clinical situations. Immune thrombocytopenic purpura (ITP) is a destructive thrombocytopenia caused by autoantibodies. Neonatal alloimmune thrombocytopenia (NATP) and post-transfusion purpura (PTP) are diseases where thrombocytopenia is caused by platelet-specific alloantibodies. HLA alloantibodies do not cause thrombocytopenia, but are commonly associated with refractoriness to platelet transfusions.

This test is designed to detect antibodies to platelet glycoproteins IIb/IIIa (HPA-1a/1b [PlA1 and PlA2], HPA-3a/3b, and HPA-4a), Ia/IIA (HPA-5a/5b), Ib/IX, and IV. In addition, this test will also detect antibodies to HLA Class I antigens (HLA-A-B).

Testing for neonatal alloimmune thrombocytopenia should be performed using a maternal serum, since platelet antibody may not be detected in a neonatal serum. False negative results are common in infant samples.

Note:
Further characterization of antibodies directed to platelet glycoproteins IIb/IIIa may be performed at client request by ordering Platelet Antibody Identification (45459).

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