T-CELLS, CD4/CD8, BY FLOW CYTOMETRY

Code
900.5137
Name
T-CELLS, CD4/CD8, BY FLOW CYTOMETRY
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
T-Cells; CD4/CD8; Lymphocyte Panel
CPT Codes
86359, 86360
Site
SBMF
Reference Test
25217
ATLAS Test Code

Specimen Information

Type

Pale yellow top (ACD) tube, WHOLE BLOOD, AND
Lavender top (EDTA) tube, WHOLE BLOOD

Volume

7.0 mL ACD whole blood, AND
5.0 mL EDTA whole blood

Transport Info

Room Temperature

Fasting Required?
False
Patient Instructions

Reference Range

See Report

Methodology

Flow Cytometry

Clinical Significance

Used to characterize some forms of immunodeficiency and autoimmune diseases.

Total T- and B-lymphocyte percentages may be used to characterize some forms of immunodeficiency and autoimmune diseases. When monoclonal antibody reagents are added to human whole blood, the flourochrome-labeled antibodies bind specifically to antigens on the surface of leukocytes. Using the CD45 antigen and side scatter, cells can be grouped and the cells of interest isolated for analysis. This method is superior to two-color methodology in that the lymphocyte gate and recovery are both assumed to be 100%.

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