VITAMIN B6 LEVEL

Code
900.5550
Name
VITAMIN B6 LEVEL
Category
None
Department
Send-Out
Start Date
Expiration Date
Synonyms
B6 Vitamin, Pyridoxal 5-Phosphate, Pyridoxal Phosphate
CPT Codes
84207
Site
SBMF
Reference Test
44322
ATLAS Test Code

Specimen Information

Type


Spec Type: Plasma - frozen
Spec Container: Green top (heparin) or light green top (PST) tube

Alt Spec Type: Serum - frozen
Alt Spec Container: Red top (serum) tube

Volume

1.0 ml

Transport Info

Promptly centrifuge 15 minutes
Immediately transfer plasma or serum to separate amber plastic tube and freeze
Spec Store Transport:
Frozen
Protect from light
Spec Stability:
After separation from cells:
Room temperature: Unacceptable
4 hours refrigerated (2-8°C)
2 months frozen (-20°C) (Avoid repeated freeze/thaw cycles)

Fasting Required?
True
Patient Instructions

Collect specimen after an overnight fast

Reference Range

20-125 nmol/L
Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.

Note:
The biologically active form of vitamin B6, pyridoxal 5-phosphate, is measured in this assay.

Methodology

Quantitative High Performance Liquid Chromatography (HPLC)

Clinical Significance

Vitamin B6 (pyridoxal 5´-phosphate, PLP) is a coenzyme involved in a variety of biochemical processes (i.e., metabolism of proteins, carbohydrates, and lipids). Dietary deficiency of vitamin B6 is uncommon. A more common cause of deficiency is the ingestion or use of antagonists such as isoniazid, hydralazine, cycloserine, and penicillamine. As deficiency progresses, electroencephalographic (EEG) changes appear that may be associated with epilepsy, especially in young patients. Skin changes include dermatitis, cheilosis, and glossitis. Sideroblastic anemias have been described that are responsive to vitamin B6 administration.This assay does not distinguish between pyridoxine, pyridoxamine, and pyridoxal forms, and does not detect pyridoxic acid.

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